Pengujian Kadar Pengawet Asam Benzoat dan Asam Sorbat Pada Permen Keras Menggunakan Kromatografi Cair Kinerja Tinggi
Determination of Benzoic Acid And Sorbic Acid Preservative Levels In Hard Candies Using High-Performance Liquid Chromatography
Abstract
Hard candy is a type of solid snack made from sugar or a mixture of other sweeteners with or without the addition of other food ingredients and food additives (BTP). Based on the BPOM Regulation No. 11 of 2019 concerning BTP, the use of benzoic acid in hard candy is a maximum of 500 mg/kg body weight while sorbic acid is a maximum of 1000 mg/kg body weight. Excessive consumption of benzoic acid and sorbic acid can endanger health. Food quality control of the use of BTP can be done by routine testing in the laboratory. One of the instruments that can detect BTP is High Performance Liquid Chromatography (HPLC). This study aims to analyze the levels of benzoic acid and sorbic acid in hard candy samples using HPLC instrument. The results of the analysis of benzoic acid and sorbic acid levels in hard candy samples showed that both were not detected by the detector. The LOD value of benzoic acid obtained on HPLC instrument was 4.92 mg/kg and the LOD of sorbic acid was 3.70 mg/kg) which limits its use to meet BPOM regulation No. 11 of 2019. The testing process for benzoic acid and sorbic acid in hard candy samples is also correct and in accordance with the procedure, where the recovery value is in the range of 90-107%, the RPD value is smaller than the 2/3 CV Horwitz value (In benzoic acid, the RPD value is 1.02% and the CV Horwitz value is 6.42 while in sorbic acid the RPD value is 0.75% and the CV Horwitz value is 4.15) with a correlation coefficient that meets the linearity (r) requirement of 0.9999.
References
Ariantini, N. S. (2019). Analisis Kadar Zat Aditif pada Minuman Sirup Rasa Marquisa. International Journal of Applied Chemistry Research |, 1(2), 2549–3671. https://doi.org/10.23887/ijacr-undiksha
Arisman, M. B. (2009). Keracunan Makanan Buku Ajar Ilmu Gizi. EGC.
Aulia, S., Savira, & Sopyan, I. (2016). Penetapan Kadar Simvastatin Menggunakan Kromatorafi Cair Kinerja Tinggi (KCKT) :Review. Farmaka, 14(2), 70–78.
BPOM. (2019). Peraturan Badan Pengawas Obat dan Makanan Nomor 11 tahun 2019 tentang Bahan Tambahan Pangan.
Chawla, G., & Ranjan, C. (2016). Principle, Instrumentation, and Applications of UPLC: A Novel Technique of Liquid Chromatography. Open Chemistry Journal, 3(1), 1–16. https://doi.org/10.2174/1874842201603010001
FDA. (2001). Guidance for industry: bioanalytical method validation. Http://Www. Fda. Gov/Cder/Guidance/4252fnl. Pdf.
Hadisutjipto, R. (2020). Validasi Metode Bioanalisis Karbamazepin dan Karbamazepin 10,11-Epoksida dalam Spiked-Plasma Menggunakan Kromatografi Cair Kinerja Tinggi (KCKT) dengan Detektor Photodiode Array. Yogyakarta.
Hilda, N. (2015). Pengaruh Pengawet Benzoat terhadap Kerusakan Ginjal. Jurnal Keluarga Sehat Sejahtera, 13(26), 14–21.
Kemenkes. (2020). Farmakope Indonesia Edisi VI. Katalog Dalam Terbitan. Kementerian Kesehatan RI Indonesia.
Nurdiani, C. U., & Ismail, M. D. (2019). Penetapan Kadar Asam Benzoat pada Margarin Bermerk Kemasan dan Bermerk Curah di Wilayah Pasar Cisalak Depok. Anakes : Jurnal Ilmiah Analis Kesehatan, 5(2), 183–188. https://doi.org/10.37012/anakes.v5i2.346
Pakpahan, A. R. (2005). Penetapan Kadar Asam Benzoat Dalam Minuman Bersoda Secara Kromatografi Cair Kinerja Tinggi.
Putra, A. Y., Mairizki, F., & Suyani, H. (2012). Penentuan Asam Benzoat, Natrium Sakarin, dan Secara Kromatografi cair Kinerja Tinggi. Jurnal Litbang Industri, 2(2), 79–86.
Ramadhan. (2012). Universitas Indonesia Pembuatan Permen Hard Candy Yang Mengandung Propolis Sebagai Permen Kesehatan Gigi.
Rohmah, S. A. A., Muadifah, A., & Martha, R. D. (2021). Validasi Metode Penetapan Kadar Pengawet Natrium Benzoat pada Sari Kedelai di Beberapa Kecamatan di Kabupaten Tulungagung Menggunakan Spektrofotometer Uv-Vis. Jurnal Sains Dan Kesehatan, 3(2), 120–127. https://doi.org/10.25026/jsk.v3i2.265
Rohman, A. (2018). Analisis Makanan. UGM PRESS.
Siswanto, A., Fudholi, A., Nugroho, A. K., & Martono, S. (2016). Validasi Metode HPLC untuk Penetapan Aspirin dan Asam Salisilat dalam Plasma Kelinci ( Lepus curpaeums ) secara Simultan Validation of A High Performance Liquid Chromatography Method for The Simultaneous Determination of Aspirin and Salisylic Acid In Rabb. Jurnal Kefarmasian Indonesia, 6(2), 68–78.
Susrama, I. G. K. (2017). E-Jurnal Agroekoteknologi Tropika. E-Jurnal Agroekoteknologi Tropika, 6(3), 310.
Tahir, M., & Nurmawati, J. S. (2019). Identifikasi Pengawet dan Pewarna Berbahaya pada Bumbu Giling yang Diperjualbelikan di Pasar Daya Makassar. Jurnal Media Laboran, 9(1), 22–28.
This work is licensed under a Creative Commons Attribution 4.0 International License.
