Evaluasi Antioksidan Minuman Kopi yang Dikombinasikan dengan Ekstrak Gambir dan Madu

Antioxidant Evaluation of Coffee Beverages Combined with Gambier Extract and Honey

Authors

  • Syerina Raihatul Jannah, S.TP., M.Sc. Polytechnic of Sriwijaya State https://orcid.org/0000-0002-0989-9222
  • Endang Verawati Politeknik Negeri Sriwijaya
  • Nancy Eka Putri Manurung Politeknik Negeri Sriwijaya
  • Onne Akbar Nur Ichsan Politeknik Negeri Sriwijaya

DOI:

https://doi.org/10.30812/jtmp.v4i1.5094

Keywords:

Antioxidant, Coffee, Free radicals, Gambier extract, Honey

Abstract

Free radicals are molecules with unpaired electrons that can trigger various serious diseases. Therefore, antioxidant intake through food and beverages is essential to prevent free radical-induced damage. This study aimed to analyze the effect of coffee, gambir extract, and honey on antioxidant levels to support health improvement. A completely randomized design (CRD) with a single factor, nonfactorial method was used, focusing on different ratios of coffee powder combined with gambir extract and honey. Five treatment formulations were tested: 100% coffee (control) and combinations with 2.5%, 5%, 7.5%, and 10% of gambir extract and honey. Each treatment was replicated three times. The observed parameters included pH, solubility, total phenol content, and antioxidant activity (IC50 value). The results showed that the combination significantly affected all the observed parameters. The values obtained ranged from pH 6.21 to 6.33, solubility from 27.40% to 31.72%, total phenol from 8.40 mg / ml to 78.53 mg / ml and IC50 from 117.89 ppm to 563.06 ppm. Therefore, the conclusion of this study is that the addition of gambir extract and honey can enhance antioxidant activity and increase the inhibition of free radicals in coffee beverages. This research is expected to contribute to the development of functional drinks.

References

Ahmed, S., Sulaiman, S. A., Baig, A. A., Ibrahim, M., Liaqat, S., Fatima, S., Jabeen, S., Shamim, N., & Othman, N. H. (January, 2018). Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action. 2018. https://doi.org/10.1155/2018/8367846.

Awwad, S., Issa, R., Alnsour, L., Albals, D., & Al-Momani, I. (December, 2021). Quantification of caffeine and chlorogenic acid in green and roasted coffee samples using hplc-dad and evaluation of the effect of degree of roasting on their levels. Molecules, 26(24). https://doi.org/10.3390/molecules26247502.

Bernatoniene, J. & Kopustinskiene, D. M. (2018). The role of catechins in cellular responses to oxidative stress. Molecules, 23(4), 965. https://doi.org/10.3390/molecules23040965.

Calvo-Rodriguez, M., Kharitonova, E. K., Snyder, A. C., Hou, S. S., Sanchez-Mico, M. V., Das, S., Fan, Z., Shirani, H., Nilsson, K. P. R., Serrano-Pozo, A., & Bacskai, B. J. (2024). Real-time imaging of mitochondrial redox reveals increased mitochondrial oxidative stress associated with amyloid β aggregates in vivo in a mouse model of Alzheimer’s disease. Molecular Neurodegeneration, 19(1). https://doi.org/10.1186/s13024-024-00702-2.

Chandimali, N., Bak, S. G., Park, E. H., Lim, H.-J., Won, Y.-S., Kim, E.-K., Park, S.-I., & Lee, S. J. (2025). Free radicals and their impact on health and antioxidant defenses: a review. Cell Death Discovery, 11(1), 19. http://dx.doi.org/10.1038/s41420-024-02278-8.

Cheng, K., Dong, W., Long, Y., Zhao, J., Hu, R., Zhang, Y., & Zhu, K. (Agustus, 2019). Evaluation of the impact of different drying methods on the phenolic compounds, antioxidant activity, and in vitro digestion of green coffee beans. Food Science and Nutrition, 7(3). https://doi.org/10.1002/fsn3.948.

Framida, F., Santoso, B., Wijaya, A., & Priyanto, G. (2023). Penambahan Gula Aren dan Krim dalam Kopi Gambir. 10(1), 368–387.

Griendling, K. K., Camargo, L. L., Rios, F. J., Alves-Lopes, R., Montezano, A. C., & Touyz, R. M. (2021). Oxidative stress and hypertension. Circulation research, 128(7), 993–1020. https://doi.org/10.1161/CIRCRESAHA.121.318063.

Guenaoui, N., Ouchemoukh, S., Amessis-Ouchemoukh, N., Ayad, R., Moumeni, L., Zeroual, B., Hadjal, S., & Plazzotta, S. (2025). Effect of honey addition on physical properties, oxidative stability, and digestibility of margarine. Food Chemistry, 470, 142692. https://doi.org/10.1016/j.foodchem.2024.142692.

Kim, Y. K., Lim, J.-M., Kim, Y. J., & Kim, W. (2024). Alterations in pH of coffee bean extract and properties of chlorogenic acid based on the roasting degree. Foods, 13(11), 1757. https://doi.org/10.3390/foods13111757.

Kowalski, S., Kopuncov´a, M., Ciesarov´a, Z., & Kukurov´a, K. (2017). Free amino acids profile of Polish and Slovak honeys based on LC–MS/MS method without the prior derivatisation. Journal of Food Science and Technology, 54(11). https://doi.org/10.1007/s13197-017-2838-7.

Liang, N. & Kitts, D. D. (2015). Role of chlorogenic acids in controlling oxidative and inflammatory stress conditions. Nutrients, 8(1), 16. https://doi.org/10.3390/nu8010016.

Machado De-Melo, A. A., de Almeida-Muradian, L. B., Sancho, M. T., & Pascual-Mat´e, A. (2018). Composici´on y propiedades de la miel de Apis mellifera: una revisi ´on. Journal of Apicultural Research, 57(1), 5–37.

Maesaroh, K., Kurnia, D., & Al Anshori, J. (2018). Perbandingan metode uji aktivitas antioksidan DPPH, FRAP dan FIC terhadap asam askorbat, asam galat dan kuersetin. Chimica et natura acta, 6 (2), 93-100. https://doi.org/10.24198/cna.v6.n2.19049.

Melia, S., Novia, D., & Juliyarsi, I. (2015). Antioxidant and antimicrobial activities of gambir (Uncaria gambir Roxb) extracts and their application in rendang. Pakistan Journal of Nutrition, 14(12). https://doi.org/10.3923/pjn.2015.938.941.

Mursalin, M., Nizori, A., & Rahmayani, I. (2019). The Effect of Heating Schedule on PhysicoChemical Properties of Instant Coffee of Liberika Tungkal Jambi. Indonesian Food Science and Technology Journal, 2(2). https://doi.org/10.22437/ifstj.v2i2.9442.

Neha, K., Haider, M. R., Pathak, A., & Yar, M. S. (2019). Medicinal prospects of antioxidants: A review. 178. https://doi.org/10.1016/j.ejmech.2019.06.010.

O’callaghan, F., Muurlink, O., & Reid, N. (2018). Risk Management and Healthcare Policy Dovepress effects of caffeine on sleep quality and daytime functioning. Risk Management and Healthcare Policy. https://doi.org/10.2147/RMHP.S156404.

Odˇzakovic, B., Dˇzinic, N., Kukric, Z., & Grujic, S. (2016). Effect of roasting degree on the antioxidant activity of different Arabica coffee quality classes. Acta Scientiarum Polonorum, Technologia Alimentaria, 15(4). https://doi.org/10.17306/J.AFS.2016.4.39.

Olszowy-Tomczyk, M. (2021). How to express the antioxidant properties of substances properly? 75(12). https://doi.org/10.1007/s11696-021-01799-1.

Patruica, S., Alexa, E., Obistioiu, D., Cocan, I., Radulov, I., Berbecea, A., Lazar, R. N., Simiz, E., Vicar, N. M., Hulea, A., & Moraru, D. (2022). Chemical Composition, Antioxidant and Antimicrobial Activity of Some Types of Honey from Banat Region, Romania. Molecules, 27(13). https://doi.org/10.3390/molecules27134179.

Perdani, C. G., Pranowo, D., & Qonitatilah (2019). Total phenols content of green coffee (Coffea arabica and Coffea canephora) in East Java. In IOP Conference Series: Earth and Environmental Science, volume 230. https://doi.org/10.1088/1755-1315/230/1/012093.

Purnawan, R. B., Bimantio, M. P., & Ulfah, M. (2025). Pembuatan Serbuk Minuman Fungsional dengan Variasi Perbandingan Kopi Arabika Honey dan Kopi Pinang Muda dengan Penambahan Ekstrak Gambir (Ucaria Gambir Roxb). Jurnal Teknologi Pangan dan Gizi (Journal of Food Technology and Nutrition), 24(1), 22–30. https://doi.org/10.33508/jtpg.v24i1.5797.

Putri, E. Y. E., Santoso, B., & Wijaya, A. (2021). Minuman fungsional instan dengan kombinasi kopi robusta (Coffea canephora), ekstraksi gambir (Uncaria gambir Roxb.) dan ginseng (Panax quinquefolius L.). 9(2021), 89–100.

Santoso, B., Aulia, M. R. W., Jannah, S. R., Priyanto, G., Wijaya, A., & Hermanto, H. (2022). Incorporation of catechin extracts from gambier products and pasak bumi in the production of functional instant green robusta coffee. Potravinarstvo Slovak Journal of Food Sciences, 16. https://doi.org/10.5219/1735.

Santoso, B., Hazirah, R., Priyanto, G., Hermanto, & Sugito (2019). Utilization of uncaria gambir roxb filtrate in the formation of bioactive edible films based on corn starch. Food Science and Technology (Brazil), 39(4). https://doi.org/10.1590/fst.06318.

Singh, N. (2021). Synthesis and characterization of chitosan prepared from shrimp shell. 6(6).

Wang, D., Yan, Z., Ren, L., Jiang, Y., Zhou, K., Li, X., Cui, F., Li, T., & Li, J. (May, 2025a). Carbon dots as new antioxidants: Synthesis, activity, mechanism and application in the food industry. Food Chemistry, 475, 143377. https://doi.org/10.1016/j.foodchem.2025.143377.

Wang, L., Song, J., Yang, Z., Zhang, H., Wang, Y., Liu, J., Wang, S., Shi, J., & Tong, X. (2025b). SERCA2 dysfunction accelerates angiotensin II-induced aortic aneurysm and atherosclerosis by induction of oxidative stress in aortic smooth muscle cells. Journal of Molecular and Cellular Cardiology, 200, 68–81. https://doi.org/10.1016/j.yjmcc.2025.01.009.

Yuliati, F. N. & Ali, H. M. (2019). Effects honey on different levels of antioxidant activity and chemical of pasteurized eggs. 247(1), 12067. https://doi.org/10.1088/1755-1315/247/1/012067.

Zaric, B. L., Macvanin, M. T., & Isenovic, E. R. (2023). Free radicals: relationship to human diseases and potential therapeutic applications. The International Journal of Biochemistry & Cell Biology, 154, 106346. https://doi.org/10.1016/j.biocel.2022.106346.

Zhang, H., Qi, G., Wang, K., Yang, J., Shen, Y., Yang, X., Chen, X., Yao, X., Gu, X., & Qi, L. (2023). Oxidative stress: roles in skeletal muscle atrophy. Biochemical Pharmacology, 214, 115664. https://doi.org/10.1016/j.bcp.2023.115664.

Published

2025-07-14

How to Cite

Evaluasi Antioksidan Minuman Kopi yang Dikombinasikan dengan Ekstrak Gambir dan Madu: Antioxidant Evaluation of Coffee Beverages Combined with Gambier Extract and Honey. (2025). Jurnal Teknologi Dan Mutu Pangan, 4(1), 10-18. https://doi.org/10.30812/jtmp.v4i1.5094