Profil Fitokimia Dan Aktivitas Antibakteri Bunga Kekara Laut (Canavalia rosea) Menggunakan Metode Difusi Cakram

Abstract

Bacterial infection is one of the leading causes of chronic infections and ongoing risks of mortality that continuously threaten public health globally. Improper use of antibiotics can lead to pathogenic bacteria developing resistance to antibiotics and cause dangerous side effects. Therefore, it is important to search for alternative antibiotic sources derived from natural materials. One type of plant that has the potential as an antibacterial agent is Canavalia rosea. This research aims to qualitatively assess the phytochemical content using tube methods and to evaluate the antibacterial activity of the ethyl acetate fraction of Canavalia rosea extract using the disc diffusion method. Extraction of jarak pagar leaves was carried out by maceration method using 96% ethanol, then fractionation was done using ethyl acetate. Phytochemical analysis results showed the presence of alkaloid, saponin, flavonoid, polyphenol, and tannin compounds in the jarak pagar leaf extract. The antibacterial activity of the ethyl acetate fraction of the extract showed significant inhibition zones against the growth of S. aureus bacteria. At a concentration of 9 mg/mL, the inhibition zone reached 20.31 ± 0.27 mm, falling into the moderate to strong category, while at a concentration of 1.5 mg/mL, the inhibition zone reached 5.13 ± 0.32 mm, indicating lower inhibitory effects. Statistical analysis showed that the ethyl acetate fraction of Canavalia rosea extract has significant antibacterial activity, with each concentration having different effects in inhibiting the growth of S. aureus.