Uji Analgetik Kombinasi Ekstrak Daun Pepaya (Carica papaya L.) dan Daun Kelor (Moringa oleifera L.) pada Mencit Putih (Mus musculus) dengan Uji Geliat

Arifina Fahamsya; Endang Istriningsih; Ananda Kukuh Adi Karang

doi:10.30812/biocity.v4i1.5194

Authors

Arifina Fahamsya Universitas Bhamada Slawi
Endang Istriningsih Universitas Bhamada Slawi
Ananda Kukuh Adi Karang Universitas Bhamada Slawi

DOI:

https://doi.org/10.30812/biocity.v4i1.5194

Keywords:

Analgesic, Mefenamic acid, Moringa leaves, Papaya leaves, Writhing test

Abstract

Ethnomedicinal plants that have been proven as analgesics are moringa leaves (Moringa oleifera L.) and papaya leaves (Carica papaya L.). The purpose of this study is to determine the analgesic activity of a combination of papaya and moringa leaf extracts. Analgesic testing used the writhing method. A total of 25 mice were grouped into 5 treatment groups, namely negative control (CMC Na 1%), positive control (mefenamic acid 500mg/70kgBW), group I (moringa leaf extract 400mg/kgBW), group II (papaya leaf extract 600mg/kgBW), and group III (combination of moringa leaf extract 200mg/kgBW and papaya leaf extract 300mg/kgBW). The results of the study were obtained using the formula for calculating the percentage of analgesic power and tested using the Post Hoc test and the Least Significant Difference (LSD) test. Based on the analysis, a significant difference was obtained between the treatment group and the negative control. The highest percentage of analgesic effect was obtained from group III, the combination of moringa leaf extract and papaya leaf extract at 56.4%. This result is equivalent to the positive control, with the administration of mefenamic acid.

References

[1] S. Sarno, “Pemanfaatan Tanaman Obat (Biofarmaka) Sebagai Produk Unggulan Masyarakat Desa Depok Banjarnegara,” Abdimas Unwahas, vol. 4, no. 2, pp. 73–78, 2019, doi: 10.31942/abd.v4i2.3007.

[2] Emilda, Hidayah Muslihatul, and Heriyati, “Analisis Pengetahuan Masyarakat Tentang Pemanfaatan Tanaman Obat Keluarga (Studi Kasus Kelurahan Situgede, Kecamatan Bogor Barat),” Anal. Pengetah., vol. 14, no. 1, pp. 11–21, 2017, doi: 10.31851/sainmatika.v14i1.1106.

[3] T. L. Schwinghammer, J. T. DiPiro, V. L. Ellingrod, and C. V. DiPiro, Dipiro, J. T., DiPiro, C. V., Schwinghammer, T. L., & Wells, B. G. (2015). Pharmacotherapy handbook. McGraw-hill. 2021.

[4] W. Dwika, P. Putra, A. Agung, G. Oka Dharmayudha, and L. M. Sudimartini, “Identifikasi Senyawa Kimia Ekstrak Etanol Daun Kelor (Moringa oleifera L) Di Bali,” Indones. Med. Veterinus Oktober, vol. 5, no. 5, pp. 464–473, 2016.

[5] A. E. Putri, E. M. ‘Alimah, and D. P. Tilarso, “Uji Efektivitas Analgetik Kombinasi Ekstrak Daun Pepaya dan Daun Kelor pada Mencit Galur Swiss Webster dengan Metode Hot Plate,” Pharmasipha Pharm. J. Islam. Pharm., vol. 7, no. 2, pp. 93–99, 2024, doi: 10.21111/pharmasipha.v7i2.10488.

[6] M. Amira, Nurul, “Antioxidant Analysis of Different Parts of Carica papaya,” vol. 20, no. 3, pp. 1043–1048, 2013.

[7] T. Sentat and S. Pangestu, “Uji Efek Analgesik Ekstrak Etanol Daun Kersen (Muntingia calabura L.) pada Mencit Putih Jantan (Mus musculus) dengan Induksi Nyeri Asam Asetat,” J. Ilm. Manuntung, vol. 2, no. 2, pp. 147–153, 2017, doi: 10.51352/jim.v2i2.59.

[8] R. Afrianti, R. Yenti, and D. Meustika, “Uji Aktifitas Analgetik Ekstrak Etanol Daun Pepaya (Carica papaya L.) pada Mencit Putih Jantan yang Diinduksi Asam Asetat 1%,” J. Sains Farm. Klin., vol. 1, no. 1, p. 54, 2015, doi: 10.29208/jsfk.2014.1.1.12.

[9] A. Muqsith, “Uji Daya Analgetik Infusa Daun Kelor ( Moringae folium ) pada Mencit ( Mus musculus ) Betina,” J. Lentera, vol. 15, no. 14, pp. 59–63, 2015.

[10] A. R. Ahmad, J. Juwita, and S. A. D. Ratulangi, “Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM),” Pharm. Sci. Res., vol. 2, no. 1, pp. 1–10, 2015, doi: 10.7454/psr.v2i1.3481.

[11] Departemen Kesehatan RI, “Parameter Standar Umum Ekstrak Tumbuhan Obat.” 2000.

[12] BPOM RI, “Pedoman Pengkajian Obat Bahan Alam, Suplemen Kesehatan Dan Kosmetik,” Buku Pedoman Kaji., pp. 1–47, 2024.

[13] K. K. RI, “Inventaris Tumbuhan Obat Edisi Revisi Jilid 1,” Kementrian Kesehatan RI. pp. 35–37, 2016.

[14] S. S. D. H. Oktaviani, “Identifikasi Etanol Hasil Fermentasi Sente (Alocasia macrorrizha (L.) G.Don), Sente Wulung (Alocasia indica (Lour).Koch) dan Kimpul (Xhantosoma ningrum (Vell.)Mansf),” Proc. Natl. Acad. Sci., vol. 3, no. 1, pp. 1–15, 2015, doi: 10.30595/pji.v8i1.594.

[15] K. P. Widiatmika, Aktivitas Antiproliferasi Ekstrak Etanol Daun Kersen (Dendrophtoe pentandra L.Miq.) terhadap Kultur Sel Kanker Nasofaring (Raji Cell Line), vol. 16, no. 2. 2015.

[16] A. R. Turner, “Screening Methods in Pharmacology,” Acad. Press. New York, vol. 3, no. 1, pp. 1–15, 1965.

[17] W. Ibrahim, R. Mutia, N. Nurhayati, N. Nelwida, and B. Berliana, “Penggunaan Kulit Nanas Fermentasi dalam Ransum yang Mengandung Gulma Berkhasiat Obat terhadap Konsumsi Nutrient Ayam Broiler,” J. Agripet, vol. 16, no. 2, pp. 76–82, 2016, doi: 10.17969/agripet.v16i2.4142.

[18] O. et. a. Nurhidayati, Lailiana; Listina, “Kombinasi ekstrak daun kelor (Moringa pterygosperma Gaertn.) dan Daun Lamtoro (Laucaena leucocephalab Lmk.) sebagai analgetik pada mencit putih jantan dengan metode geliat,” vol. 4, no. 6, pp. 627–634.

[19] T. Kusumaningsih, N. J. Asrilya, S. Wulandari, D. R. T. Wardani, and K. Fatihin, “Reduction on the Levels of Tannins From Stevia rebaudiana Extract Using Activated Carbon,” ALCHEMY J. Penelit. Kim., vol. 11, no. 1, p. 81, 2015, doi: 10.20961/alchemy.v11i1.111.

[20] R. Febriyanti, A. V. Purba, and P. Simanjuntak, “Uji Aktifitas Analgetik Kombinasi Ekstrak Buah Belimbing Wuluh (Averrhoa bilimbi L.) dan Daun Seledri (Apium graveolens L.) terhadap Mencit Putih Jantan (Mus musculus) dengan Metode Geliat,” Parapemikir J. Ilm. Farm., vol. 7, no. 1, pp. 197–201, 2018, doi: 10.30591/pjif.v7i1.737.

[21] S. R. Rachmawati and J. Suriawati, “Identifikasi Senyawa Kimia dan Nilai Gizi Ekstrak Air Daun Kelor (Moringa oleifera L.) sebagai Pengawet Alami Mie Basah,” SANITAS J. Teknol. dan Seni Kesehat., vol. 10, no. 2, pp. 102–116, 2019, doi: 10.36525/sanitas.2019.11.

[22] A. Nur, “Efek Analgetik Kombinasi Ekstrak Buah Belimbing Wuluh (Averrhoa bilimbi L.) dan Ekstrak Daun Pepaya (Carica papaya L.) Pada Mencit (Mus musculus),” J. Ilm. As-Syifaa, vol. 10, no. 2, pp. 213–220, 2018, doi: 10.33096/jifa.v10i2.430.

[23] S. Meisyayati, J. Immanuel, and D. Darwis, “Efek Analgetik Kombinasi Ekstrak Daun Pepaya (Carica papaya L) dan Ekstrak Daun Asam Jawa (Tamarindus indica L) pada Mencit Putih Jantan,” J. Ilm. Bakti Farm., vol. 2, no. 1, pp. 63–70, 2017.

[24] A. Nugroho, H. Heryani, J. S. Choi, and H. J. Park, “Identification and quantification of flavonoids in Carica papaya leaf and peroxynitrite-scavenging activity,” Asian Pac. J. Trop. Biomed., vol. 7, no. 3, pp. 208–213, 2017, doi: 10.1016/j.apjtb.2016.12.009.

[25] “Uji Aktivitas Analgetik Ekstrak Etanol 96 % Daun Belimbing Wuluh (Averrhoa bilimbi L.) dengan Metode Geliat pada Mencit Putih (Mus musculus L.) Galur Swiss Webster,” vol. 2, no. 2, pp. 30–34, 2020, doi: 10.32795/widyakesehatan.v2i2.963.

[26] A. A. . Tamimi, E. De Queljoe, and J. P. Siampa, “Uji Efek Analgesik Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus),” Pharmacon, vol. 9, no. 3, p. 325, 2020, doi: 10.35799/pha.9.2020.30015.