Uji Aktivitas Antibakteri Ekstrak Daun Tempuyung (Sonchus arvensis L.) terhadap Pseudomonas aeruginosa dan Bacillus cereus

Amrina Rosada; Baiq Yulia Hasni Pratiwi; Lalu Busyairi Muhsin

doi:10.30812/biocity.v4i2.6256

Authors

Amrina Rosada Universitas Bumigora
Baiq Yulia Hasni Pratiwi Universitas Bumigora
Lalu Busyairi Muhsin Universitas Bumigora

DOI:

https://doi.org/10.30812/biocity.v4i2.6256

Keywords:

Antibacterial activity; Bacillus cereus; diffusion method; Pseudomonas aeruginosa; Sonchus arvensis L.

Abstract

Pseudomonas aeruginosa and Bacillus cereus are two dangerous pathogenic bacteria that are often resistant to antibiotics. This study aims to determine the antibacterial activity of the ethanol extract of tempuyung leaves (Sonchus arvensis L.) against Pseudomonas aeruginosa and Bacillus cereus bacteria. The antibacterial activity test used the well-diffusion method at extract concentrations of 10%, 25%, 50%, and 75%, with ciprofloxacin as the positive control and 10% DMSO as the negative control. The results of the antibacterial test showed that the extract did not produce an inhibition zone at any concentration against either bacterium, whereas the positive control showed a strong inhibition zone. Thus, the ethanol extract of tempuyung leaves was ineffective against Pseudomonas aeruginosa and Bacillus cereus under the tested conditions, with inhibition zones not exceeding the strong category. Further research is recommended to identify the active compounds using GC-MS and to test them on other types of bacteria.

References

Amal, S., Gunarti, N. S., Saragih, D. S., & Hidayah, H. (2022). Uji toksisitas akut ekstrak etanol daun tempuyung (Sonchus arvenis L.) pada mencit betina dengan metode fixed dose. Journal of Pharmacopolium, 5(2), 190–198.

Amin, S., Nisa, F. K., Setiawati, Y., & Fauzan, M. A. A. (2025). Kajian kimia medisinal ciprofloxacin: Mekanisme kerja, antibakteri, dan pola resistensi bakteri. Jurnal Ilmiah Kedokteran dan Kesehatan, 4(2), 121–131. https://doi.org/10.55606/klinik.v4i2.3923.

Amini, F., Hasanah, N. U., & Alrosyidi, A. F. (2024). Analisis penggunaan antibiotik menggunakan metode ddd (defined daily dose) pada pasien rawat jalan di puskesmas proppo kabupaten pamekasan periode januari- maret 2024. Jurnal Ilmiah Kajian Multidisipliner, 8.

Anggita, D., Nuraisyah, S., & Wiriansya, E. P. (2022). Mekanisme kerja antibiotik. UMI Medical Journal, 7(1), 46. https://doi.org/10.33096/umj.v7i1.149.

Asworo, R. Y. & Widwiastuti, H. (2023). Pengaruh ukuran serbuk simplisia dan waktu maserasi terhadap aktivitas antioksidan ekstrak kulit sirsak. Indonesian Journal of Pharmaceutical Education, 3(2). https: //doi.org/10.37311/ijpe.v3i2.19906.

Bao, L., Peng, R., Ren, X., Ma, R., Li, J., & Wang, Y. (2012). Analysis of some common pathogens and their drug resistance to antibiotics. Pakistan Journal of Medical Sciences, 29(1), 135–139. https://doi.org/ 10.12669/pjms.291.2744.

Endarini, L. H., Mutiarawati, D. T., & Nugrahini, A. (2022). Pertumbuhan bakteri bacillus cereus dan salmonella typhi pada ekstrak mentimun (cucumis sativus). Jurnal Penelitian Kesehatan Suara Forikes. https://doi.org/10.33846/sf13317.

Farmakope Herbal Indonesia (2017). Farmakope Herbal Indonesia. Direktur Jenderal Kefarmasian dan Alat Kesehatan Kementerian Kesehatan Republik Indonesia.

Harahap, N. I. (2020). Skrining dan karakterisasi simplisia daun tempuyung (sonchus arvenis. l) imelda pekerja indonesia. Jurnal Ilmiah Farmasi Imelda, 3(2), 2655–3147. https://doi.org/10.52943/ jifarmasi.v3i2.212.

Karunathilaka, R. D. N., Silva, A. R. N., Ranaweera, C. B., Dissanayake, D. M. R. K., Nelumdeniya, N. R. M., Pathirana, R., & Ratnasooriya, W. D. (2016). In vitro antibacterial activity of hexane, chloroform and methanolic extract of different parts of acronychia pedunculata grown in sri lanka. International Journal of Advanced Research, 4(8), 1574–1579. https://doi.org/10.21474/IJAR01/1364.

Li, T., Zou, Q., Chen, C., Li, Q., Luo, S., Li, Z., Yang, C., Yang, D., Huang, Z., Zhang, H., Tang, W., & Qi, L. (2023). A foodborne outbreak linked to bacillus cereus at two middle schools in a rural area of chongqing, china, 2021. PLoS ONE, 18(10). https://doi.org/10.1371/journal.pone.0293114.

Mierza, V., Antolin, A., Ichsani, A., Dwi, N., Sridevi, S., & Dwi, S. (2023). Research article: Isolasi dan identifikasi senyawa terpenoid. Jurnal Surya Medika, 9(2), 134–141. https://doi.org/10.33084/ jsm.v9i2.5681.

Pachori, P., Gothalwal, R., & Gandhi, P. (2019). Emergence of antibiotic resistance pseudomonas aeruginosa in intensive care unit; a critical review. Genes and Diseases, 6(2), 109–119. https://doi.org/10. 1016/j.gendis.2019.04.001.

Pang, Z., Raudonis, R., Glick, B. R., Lin, T. J., & Cheng, Z. (2019). Antibiotic resistance in pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnology Advances, 37(1), 177–192. https://doi.org/10.1016/j.biotechadv.2018.11.013.

Putra, B. R. S., Kusrini, D., & Fachriyah, E. (2013). Isolasi senyawa antioksidan dari fraksi etil asetat daun tempuyung (sonchus arvenis. l). Jurnal Kimia Sains dan Aplikasi, 16(3).

Rahayu, S. & Jejak Artikel, R. (2020). Induksi kalus dengan 2,4d dan bap pada eksplan daun vegetatif dan generatif tempuyung (sonchus arvensis l.). Jurnal Ilmiah Biologi Unsoed, 2(3).

Raynaldi Joel, K. & Astuty Lolo, W. (2016). Identifikasi daun uji sensitifitas bakteri yang diisolasi dari sputumpenderita pneumonia di rsup prof. dr. r. d. kandou manado terhadap antibiotik eritromisin, seftriakson dan sefadroksil. PHARMACON Jurnal Ilmiah Farmasi-UNSRAT, 5(4).

Sitorus, C. J. & Hutabarat, G. A. R. (2024). Uji kandungan alkaloid pada bubuk kayu manis (cinnamomum burmanni) dengan metode sokletasi. Jurnal Matematika dan Ilmu Pengetahuan Alam, 2. https://doi. org/10.59581/konstanta.v2i2.2367.

Wardani, Y. E. & Melati, M. (2014). Produksi simplisia dan kandungan bioaktif daun tempuyung (sonchus arvensis l.) pada berbagai dosis pupuk kandang kambing. J. Hort. Indonesia, 5(3), 148–157.

Yennie, Y., Dewanti-Hariyadi, R., Kusumaningrum, H. D., & Poernomo, A. (2022). Kontaminasi staphylococcus aureus dan bacillus cereus pada susyi tingkat retil di wilayah jabodetabek. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(2), 331–344. https://doi.org/10.17844/jphpi.v25i2.42066.

Yuliati (2024). Testing the antibacterial effectiveness of black cumin extract on the growth of bacteria bacillus cereus disk diffusion method. Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan, 18(1), 84–89. https://doi.org/10.33533/jpm.v18i1.7682.

Zhang, G. F., Liu, X., Zhang, S., Pan, B., & Liu, M. L. (2018). Ciprofloxacin derivatives and their antibacterial activities. European Journal of Medicinal Chemistry, 146, 599–612. https://doi.org/10.1016/j. ejmech.2018.01.078.