DESAIN SISTEM AUTOFOCUS BERBASIS NORMALIZED VARIANCE UNTUK PENCARI FOKUS OTOMATIS MIKROSKOP DIGITAL

Abstract

The objective of this study is to design digital microscope interface that could determine focus automatically. This digitalmicroscope is equipped with a stepper motor as an object table driver (x & y axis) and microscope focus driver (z axis).The focus is determined based on the calculation of Normalized Variance (F) from the microscope images. Higher Fvalue resulted in clearer images. In contrary, lower F value resulted in vague images. Based on those criteria, the focusof microscope will be achieved when the maximal F value is obtained. The automatic microscope focus attained wascompared with conventional method (manual) to determine its congruence with t-paired test. Based on t-paired test result,p value = 0.313 is bigger than α = 0.05, thus no difference is found between automatic focus point and conventional focuspoint. Therefore, this method could be used to replace the conventional method to determine digital microscope focus.